A COMPLEX APPROACH TO PHYTO/RHIZOREMEDIATION OF POLYCHLORINATED BIPHENYLS
The biological remediation of environment contaminated by polychlorinated biphenyls (PCB) is of worldwide interest because these compounds are now nearly ubiquitous, recalcitrant and expensive to be destroyed by physical or chemical methods. We study their removal from different aspects, separatelyPCB metabolisation in bacteria and plants, stepwisefrom aseptic plant tissues to pot and contaminated field experiments . The products formed from PCB in plants were identified, and their toxicity estimated . Genetically modified plants have been prepared bearing bacterial genes responsible for cleavage of the biphenyl ring . The studies of cooperation between plants and soil microorganisms shifted from analysis of cultivable microorganisms to the non-cultivable ones, too. This was allowed by the application of molecular genetics,improvement of sequencing speed and capacity, soil DNA extraction and metagenomic approaches. The exploitation of stable isotope probing allowed evaluating taxonomy , presence of functional genes and the effect of vegetation or isolated plant-formed compounds on composition of soil bacterial communityand its degradative abilities.
Acknowledgement: the authors thank GACR13-28283S.
Tomas, Macek ( presenting )
Department of Biochemistry and Microbiology, Faculty of Food and Biochemical Technology, ICTP, 16628 Prague, Czech Republic
Martina , Novakova
Petra , Lovecka
Contribution proposed for: oral presentation